Genotyping and primer details


Genotyping and primer details for SALK_081200c - At1g33230
Line ID SALK_081200c
Locus At1g33230 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:12049143 of line SALK_081200c Chr1:12049143
   
Insertion allele amplicon LBb3 - 35ux
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 35ux: 5'-GAAACAAAACCAACAAGACATTGA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:12049571
   
wt amplicon (over InsPos) 60vl - 35ux
wt amplicon start Chr1:12048874
wt amplicon end Chr1:12049571
wt amplicon size 698 bp
Locus specific primer 35ux: 5'-GAAACAAAACCAACAAGACATTGA-3'
2nd locus specific primer 60vl: 5'-CCACCACTATGAAATTGCTGTAAA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11