Sequences from confirmation amplicon


Confirmation sequences for line SALK_081200c and hit At1g33230 are shown.
Line ID SALK_081200c
Locus At1g33230 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:12049143
Primers used for PCR 35ux - LBb3, show primer details
   
Sequence Name 27-K117668-SALK_081200C-R210
GenBank Accession LN485071 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>27-K117668-SALK_081200C-R210
CATTTGTTTACCCACAATATATCCTGAAACTNCAAATATGGCTTTGAAAATATTTACTAA
GTAAACAAAACTTCATGATGTCCTCTTAGACTTGTAAGATGATCTGAACAATGAATGATC
TTTGCTTGCTCTCACCAGTTCAAAGGAAACTTCAGACTTATTCTAAGTTCATAAGCTTGT
TTATCATACTGTAGTTAAACATGTTTGTATTCTACTATGGAAACTCAGGGCTTTGAAGCA
TACGTGGGATTCCAGTTGCTTAGGACAGTGTTGATGGGGGTAGTCGCTGAATGGCAGGTA
TAAGTCTCTCTTCCGGCAGCACTTGCGCAGTATCTCCTCTGGTTTATTCATGCACCAATC
TGACAAAAAACATAACATAATGATGTATACAATAGTAGAATAGATATAATTGATACTGAA
AACTCAATGTCTTGTTGGTTTTGTTTCA
   
Sequence Name 64-K117669-SALK_081200C-35ux
GenBank Accession LN485856 [GenBank]
Sequencing primer gene specific primer, 35ux: 5'-GAAACAAAACCAACAAGACATTGA-3'
Sequence (A. th genome BLAST matches underlined) 
>64-K117669-SALK_081200C-35ux
ATCATTATGTTATGTTTTTTGTCAGATTGGTGCATGAATAAACCTGAGGAGATACTGCGC
AAGTGCTGCCGGAAGAGAGACTTATACCTGCCATTCAGCGACTACCCCCATCAACACTGT
CCTAAGCAACTGGAATCCCACGTATGCTTCAAAGCCCTGAGTTTCCATAGTAGAATACAA
ACATGTTTAACTACAGTATGATAAACAAGCTTATGAACTTAGAATAAGTCTGAAGTTTCC
TTTGAACTGGTGAGAGCAAGCAAAGATCATTCATTGTTCAGATCATCTTACAAGTCTAAG
AGGACATCATGAAGTTTTGTTTACTTAGTAAATATTTTCAAAGCCATATTTGAGTTTCAG
GATATATTGTGGTGTAAACAAATTGACGCTTAGACAACTTAATAACACATTGCGGACGTT
TTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCCT
TCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGGC
GAAAATCCTGTTTGATGGTGGTTCCGA

Last Updated on 06.12.2018 16:11