Genotyping and primer details


Genotyping and primer details for SALK_081332 - At4g29550
Line ID SALK_081332
Locus At4g29550 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:14502591 of line SALK_081332 Chr4:14502591
   
Insertion allele amplicon R212 - 61xs
T-DNA primer R212: 5'-CCTTAGGCGACTTTTGAACGCGC-3'
Locus specific primer 61xs: 5'-GCTCGAGTTACGGGTAACAGTTC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:14501556
   
Insertion allele amplicon R204 - BI88
T-DNA primer R204: 5'-GCGTGGACCGCTTGCTGCAACT-3'
Locus specific primer BI88: 5'-ATCCTCAGGTCTTCATCTTTTTGA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:14503198
   
wt amplicon (over InsPos) BI89 - BI88
wt amplicon start Chr4:14502223
wt amplicon end Chr4:14503198
wt amplicon size 976 bp
Locus specific primer BI88: 5'-ATCCTCAGGTCTTCATCTTTTTGA-3'
2nd locus specific primer BI89: 5'-TATTGCCTTTCTACTTGTCTCCGT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11