Genotyping and primer details


Genotyping and primer details for SALK_081563c - At4g29840
Line ID SALK_081563c
Locus At4g29840 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr4:14601052 of line SALK_081563c Chr4:14601052
   
Insertion allele amplicon LBb3 - 11ds
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 11ds: 5'-GCCCTTCTAACCTCAAACTATTCA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr4:14600084
   
wt amplicon (over InsPos) 33lt - 11ds
wt amplicon start Chr4:14600084
wt amplicon end Chr4:14601500
wt amplicon size 1417 bp
Locus specific primer 11ds: 5'-GCCCTTCTAACCTCAAACTATTCA-3'
2nd locus specific primer 33lt: 5'-TCCTCTGGATGCAGTATTTAATTG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11