Genotyping and primer details


Genotyping and primer details for SALK_081725c - At5g45180
Line ID SALK_081725c
Locus At5g45180 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:18275244 of line SALK_081725c Chr5:18275244
   
Insertion allele amplicon LBb3 - 14ke
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 14ke: 5'-TATCAAATTAGCACAACATTTCGG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:18274797
   
wt amplicon (over InsPos) 07lz - 14ke
wt amplicon start Chr5:18274797
wt amplicon end Chr5:18275744
wt amplicon size 948 bp
Locus specific primer 14ke: 5'-TATCAAATTAGCACAACATTTCGG-3'
2nd locus specific primer 07lz: 5'-ACTCCAAGTTGCAGAAAGAAAAAG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11