Genotyping and primer details


Genotyping and primer details for SALK_082525c - At1g74750
Line ID SALK_082525c
Locus At1g74750 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:28087942 of line SALK_082525c Chr1:28087942
   
Insertion allele amplicon LBb3 - 38cv
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 38cv: 5'-GAGTACAACCTTGACCAACCATTT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:28088288
   
wt amplicon (over InsPos) 19dz - 38cv
wt amplicon start Chr1:28087473
wt amplicon end Chr1:28088288
wt amplicon size 816 bp
Locus specific primer 38cv: 5'-GAGTACAACCTTGACCAACCATTT-3'
2nd locus specific primer 19dz: 5'-ACAATGTCTCTGGAAAGAGGAAAA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11