Genotyping and primer details


Genotyping and primer details for SALK_083120 - At2g36310
Line ID SALK_083120
Locus At2g36310 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:15226183 of line SALK_083120 Chr2:15226183
   
Insertion allele amplicon LBb3 - 78xo
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 78xo: 5'-AATAAGAAAATTGTGGCTGGACG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:15226890
   
Insertion allele amplicon R204 - AW33
T-DNA primer R204: 5'-GCGTGGACCGCTTGCTGCAACT-3'
Locus specific primer AW33: 5'-AGAAAGCTCCACCAAGAATAACAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:15225525
   
wt amplicon (over InsPos) AW34 - AW33
wt amplicon start Chr2:15225525
wt amplicon end Chr2:15226353
wt amplicon size 829 bp
Locus specific primer AW33: 5'-AGAAAGCTCCACCAAGAATAACAA-3'
2nd locus specific primer AW34: 5'-AAAGATTCAGGGGAAATTGTTCTT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11