Genotyping and primer details


Genotyping and primer details for SALK_083569c - At1g34120
Line ID SALK_083569c
Locus At1g34120 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:12427394 of line SALK_083569c Chr1:12427394
   
Insertion allele amplicon LBb3 - 12gq
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 12gq: 5'-TTGGGGATTGATACAAATGGTAAT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:12426467
   
wt amplicon (over InsPos) 01oy - 12gq
wt amplicon start Chr1:12426467
wt amplicon end Chr1:12427828
wt amplicon size 1362 bp
Locus specific primer 12gq: 5'-TTGGGGATTGATACAAATGGTAAT-3'
2nd locus specific primer 01oy: 5'-GAACGATTTCCTGAAGACTGAAAC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11