Genotyping and primer details


Genotyping and primer details for SALK_083793c - At2g38700
Line ID SALK_083793c
Locus At2g38700 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:16181128 of line SALK_083793c Chr2:16181128
   
Insertion allele amplicon LBb3 - 43fx
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 43fx: 5'-ATTTATCGTTGTGACACTGCTTTG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:16180206
   
wt amplicon (over InsPos) 19vb - 43fx
wt amplicon start Chr2:16180206
wt amplicon end Chr2:16181631
wt amplicon size 1426 bp
Locus specific primer 43fx: 5'-ATTTATCGTTGTGACACTGCTTTG-3'
2nd locus specific primer 19vb: 5'-ATGGATACATCTCCACCCATATTC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11