Genotyping and primer details


Genotyping and primer details for SALK_083842c - At5g20790
Line ID SALK_083842c
Locus At5g20790 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:7040070 of line SALK_083842c Chr5:7040070
   
Insertion allele amplicon R204 - BL01
T-DNA primer R204: 5'-GCGTGGACCGCTTGCTGCAACT-3'
Locus specific primer BL01: 5'-AATATGTTCGGTTTTCATTTTTGC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:7039408
   
wt amplicon (over InsPos) BL02 - BL01
wt amplicon start Chr5:7039408
wt amplicon end Chr5:7040439
wt amplicon size 1032 bp
Locus specific primer BL01: 5'-AATATGTTCGGTTTTCATTTTTGC-3'
2nd locus specific primer BL02: 5'-CGGTTCTAAATCTGAAAACGAATC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11