Genotyping and primer details


Genotyping and primer details for SALK_084244c - At1g16180
Line ID SALK_084244c
Locus At1g16180 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:5541235 of line SALK_084244c Chr1:5541235
   
Insertion allele amplicon LBb3 - 88by
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 88by: 5'-CATGCATGATAAACAGAAGGAGAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:5540334
   
wt amplicon (over InsPos) 32lt - 88by
wt amplicon start Chr1:5540334
wt amplicon end Chr1:5541836
wt amplicon size 1503 bp
Locus specific primer 88by: 5'-CATGCATGATAAACAGAAGGAGAA-3'
2nd locus specific primer 32lt: 5'-ACTACAATGGCAAAGACGAATACA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11