Genotyping and primer details


Genotyping and primer details for SALK_084636 - At1g20370
Line ID SALK_084636
Locus At1g20370 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:7053597 of line SALK_084636 Chr1:7053597
   
Insertion allele amplicon LBb3 - 20ow
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 20ow: 5'-CCATTCGTAATTTCTTGGTTTGTT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:7053259
   
wt amplicon (over InsPos) 06px - 20ow
wt amplicon start Chr1:7053259
wt amplicon end Chr1:7054046
wt amplicon size 788 bp
Locus specific primer 20ow: 5'-CCATTCGTAATTTCTTGGTTTGTT-3'
2nd locus specific primer 06px: 5'-ATCTAAAATGCAACTGGTCGAACT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11