Genotyping and primer details


Genotyping and primer details for SALK_084914c - At1g72810
Line ID SALK_084914c
Locus At1g72810 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:27400327 of line SALK_084914c Chr1:27400327
   
Insertion allele amplicon LBb3 - 54cy
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 54cy: 5'-TTTATCCGCAGGTAAGAAGACAAT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:27399564
   
wt amplicon (over InsPos) 20dz - 54cy
wt amplicon start Chr1:27399564
wt amplicon end Chr1:27400648
wt amplicon size 1085 bp
Locus specific primer 54cy: 5'-TTTATCCGCAGGTAAGAAGACAAT-3'
2nd locus specific primer 20dz: 5'-TCATGAACGTCAAAGATTCATTTC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11