Genotyping and primer details


Genotyping and primer details for SALK_085292c - At1g76090
Line ID SALK_085292c
Locus At1g76090 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:28551658 of line SALK_085292c Chr1:28551658
   
Insertion allele amplicon LBb3 - 61cs
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 61cs: 5'-CAATAGCAGAAAGAATCACAACCA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:28550732
   
wt amplicon (over InsPos) 21dz - 61cs
wt amplicon start Chr1:28550732
wt amplicon end Chr1:28551790
wt amplicon size 1059 bp
Locus specific primer 61cs: 5'-CAATAGCAGAAAGAATCACAACCA-3'
2nd locus specific primer 21dz: 5'-CTGGATTATTCCCACAATACACCT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11