Genotyping and primer details


Genotyping and primer details for SALK_085482c - At3g28920
Line ID SALK_085482c
Locus At3g28920 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:10941156 of line SALK_085482c Chr3:10941156
   
Insertion allele amplicon LBb3 - 36ux
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 36ux: 5'-ACTCCCTATTACAGGTCCCACTTT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:10941944
   
wt amplicon (over InsPos) 75vl - 36ux
wt amplicon start Chr3:10941002
wt amplicon end Chr3:10941944
wt amplicon size 943 bp
Locus specific primer 36ux: 5'-ACTCCCTATTACAGGTCCCACTTT-3'
2nd locus specific primer 75vl: 5'-CGAAGTTTAGATCTGAGAACGGTG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11