Sequences from confirmation amplicon


Confirmation sequences for line SALK_085482c and hit At3g28920 are shown.
Line ID SALK_085482c
Locus At3g28920 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:10941156
Primers used for PCR 36ux - LBb3, show primer details
   
Sequence Name 30-K117668-SALK_085482C-R210
GenBank Accession LN485138 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>30-K117668-SALK_085482C-R210
TCATTTGTTTACCACAATATATACTCAATTGCGGCGGTTGTTGTTGAAGATGGAAGAAGA
GACGGTGGTGGAACGGCGGAGGAATCGTCAGTTTCACGGCGGTGGAAGTTACGGTGACAA
CCACAAGCTGCACACTTGAGAGAAGTTGGATCGGAAGGTGTTGACGAAGGAGACGGCATA
AATTCGCCGCAACCGTCAAGCGCGTGACCACCAATCGCCGCCGCGTGGTTCTTGAGACAT
TCTTTGTAAGTGACTTTGTTGTTGTTGTGGTGGTGGTGGTGGTGGCGTTTGATGATTCCG
TTACTGAAAGAGATTGGTTTAGCTGGTTGGATCCGGGTCGGAGTTTCGGATTCGGGTTCT
GGTGATTTTGGAGTCATATCCATTGATCTAACTTCAAGCATCTTTATGTTTTTATTTTCT
TGAAAAAAATCAAGAAAATATTAGGGTTTATGTTAAGCTTACAAAAAAAGAAAAGTTTGA
TATTGAGAAATGAAGAAGAAGATGGATAAAAAGAGATTAAATCAACTTTTGAAGATCATG
TTTTTTGTAATAAGTTTAAGAAAATAAACTTTAGAGGAAGAAGATGTGTTATAAGATGAT
GATAGTGAATCTGCTCTGGTTGATGAATGTTGCTAAGAGAGAATAAAATAATTTAATAGG
GAGAGATATAAAAATGTTAATAAATGTGAATAATAAATATATT
   
Sequence Name 67-K117669-SALK_085482C-36ux
GenBank Accession LN485938 [GenBank]
Sequencing primer gene specific primer, 36ux: 5'-ACTCCCTATTACAGGTCCCACTTT-3'
Sequence (A. th genome BLAST matches underlined) 
>67-K117669-SALK_085482C-36ux
TCAACCCTACCTTTCTCTGTTAACATCTTCAAGCTCTGTCCTTTTATTTTAAATATATTT
ATTATTCACATTTATTAACATTTTTATATCTCTCCCTATTAAATTATTTTATTCTCTCTT
AGCAACATTCATCAACCAGAGCAGATTCACTATCATCATCTTATAACACATCTTCTTCCT
CTAAAGTTTATTTTCTTAAACTTATTACAAAAAACATGATCTTCAAAAGTTGATTTAATC
TCTTTTTATCCATCTTCTTCTTCATTTCTCAATATCAAACTTTTCTTTTTTTGTAAGCTT
AACATAAACCCTAATATTTTCTTGATTTTTTTCAAGAAAATAAAAACATAAAGATGCTTG
AAGTTAGATCAATGGATATGACTCCAAAATCACCAGAACCCGAATCCGAAACTCCGACCC
GGATCCAACCAGCTAAACCAATCTCTTTCAGTAACGGAATCATCAAACGCCACCACCACC
ACCACCACAACAACAACAAAGTCACTTACAAAGAATGTCTCAAGAACCACGCGGCGGCGA
TTGGTGGTCACGCGCTTGACGGTTGCGGCGAATTTATGCCGTCTCCTTCGTCAACACCTT
CCGATCCAACTTCTCTCAAGTGTGCAGCTTGTGGTTGTCACCGTAACTTCCACCGCCGTG
AAACTGACGATTCCTCCGCCGTTCCACCACCGTCTCTTCTTCCATCTTCAACAACAACCG
CCGCAATTGAGTATATATTGTGGTGTAAACAAATTGACGCTTAGACAACTTAATAACACA
TTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAG
CTGATTGGGCCTTCACGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGGTTG
GCCCAGCAGGCGAAAATCCTGTTTGATGGTGGT

Last Updated on 06.12.2018 16:11