Genotyping and primer details


Genotyping and primer details for SALK_085678 - At1g64990
Line ID SALK_085678
Locus At1g64990 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:24142126 of line SALK_085678 Chr1:24142126
   
Insertion allele amplicon LBb3 - 43wu
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 43wu: 5'-TGAAGAGACTGCAAAAATAGAGCA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:24141656
   
wt amplicon (over InsPos) 16xf - 43wu
wt amplicon start Chr1:24141656
wt amplicon end Chr1:24142951
wt amplicon size 1296 bp
Locus specific primer 43wu: 5'-TGAAGAGACTGCAAAAATAGAGCA-3'
2nd locus specific primer 16xf: 5'-TTTCTGAGTGGGTACCTGAGTTCT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11