Genotyping and primer details


Genotyping and primer details for SALK_085849c - At3g22970
Line ID SALK_085849c
Locus At3g22970 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:8152864 of line SALK_085849c Chr3:8152864
   
Insertion allele amplicon LBb3 - 51vi
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 51vi: 5'-TCATTGACGATCTTCTTCATATCG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:8153197
   
wt amplicon (over InsPos) 61vw - 51vi
wt amplicon start Chr3:8152307
wt amplicon end Chr3:8153197
wt amplicon size 891 bp
Locus specific primer 51vi: 5'-TCATTGACGATCTTCTTCATATCG-3'
2nd locus specific primer 61vw: 5'-TATTCTTTGCTTCGTTTTTGTGAA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11