Genotyping and primer details


Genotyping and primer details for SALK_086131 - At5g45710
Line ID SALK_086131
Locus At5g45710 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:18542746 of line SALK_086131 Chr5:18542746
   
Insertion allele amplicon LBb3 - 27kh
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 27kh: 5'-AGAAATGGGAATTCTTGAATGATG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:18541907
   
wt amplicon (over InsPos) 08mc - 27kh
wt amplicon start Chr5:18541907
wt amplicon end Chr5:18542753
wt amplicon size 847 bp
Locus specific primer 27kh: 5'-AGAAATGGGAATTCTTGAATGATG-3'
2nd locus specific primer 08mc: 5'-TCTCAGTGGAACCAAAAGAAAACT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11