Genotyping and primer details


Genotyping and primer details for SALK_086199c - At5g06560
Line ID SALK_086199c
Locus At5g06560 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:2003757 of line SALK_086199c Chr5:2003757
   
Insertion allele amplicon LBb3 - 13gq
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 13gq: 5'-TGATGGAGCTTTAGTCTTTTGGAT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:2002995
   
wt amplicon (over InsPos) 07il - 13gq
wt amplicon start Chr5:2002995
wt amplicon end Chr5:2003901
wt amplicon size 907 bp
Locus specific primer 13gq: 5'-TGATGGAGCTTTAGTCTTTTGGAT-3'
2nd locus specific primer 07il: 5'-ATCGAACCTGAGTTTTGAGTTTGT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11