Genotyping and primer details


Genotyping and primer details for SALK_087012c - At3g59060
Line ID SALK_087012c
Locus At3g59060 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:21829738 of line SALK_087012c Chr3:21829738
   
Insertion allele amplicon LBb3 - AD12
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer AD12: 5'-AGCTAAATCTTCCATCCATTCAGA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:21830219
   
wt amplicon (over InsPos) 12cd - AD12
wt amplicon start Chr3:21829433
wt amplicon end Chr3:21830219
wt amplicon size 787 bp
Locus specific primer AD12: 5'-AGCTAAATCTTCCATCCATTCAGA-3'
2nd locus specific primer 12cd: 5'-CGAAGAGAAGAAATGAGAGGAGAA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11