Sequences from confirmation amplicon


Confirmation sequences for line SALK_087012c and hit At3g59060 are shown.
Line ID SALK_087012c
Locus At3g59060 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:21829738
Primers used for PCR AD12 - LBb3, show primer details
   
Sequence Name 50-K107479-SALK_087012C-AD12
GenBank Accession LN485499 [GenBank]
Sequencing primer gene specific primer, AD12: 5'-AGCTAAATCTTCCATCCATTCAGA-3'
Sequence (A. th genome BLAST matches underlined) 
>50-K107479-SALK_087012C-AD12
TTTATGCTTCCCTTAGTAAGAGTTTACTATATATAGAATTAGTTGCTTTATTAAATCATT
TCCTCCTAGATTGTTGGAGAGGGTTGTTTGGTTTAATTATTTTCTAATCATTAAACTGTT
GGGATTTCGAATAATCTTTTCTAAGTTGATTGCTTTCATCATCCTTTTTCCTCAAATCTC
CTGTTAATCATTATACTAATTATGAATCAAATCATTTCTTATTCACTTACAAAAAAGTAA
TATATTCTTTATTTAAGTGTCTTTACAGATCTGACATGGAACAAGTGTTTGCTGATTGGA
ATTTTGAAGATAATTTTCACATGTCCACTAATAAAAGATCAATCAGGTATATATCAATTT
TCCAATTCAAGTATTTCATAATTATATACCAATTTCACATCTTAATCATAGCTATTTAAT
ATGTTTGTTTACATTATTTGTCCACAATATATTGTGGTGTAAACAAATTGACGCTTAGAC
AACTTAATAACACATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGT
GAGACGGGCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGG
TCCACGCTGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTCCGAATCNGGCA
AA
   
Sequence Name 66-K107480-SALK_087012C-LBb3
GenBank Accession LN485890 [GenBank]
Sequencing primer T-DNA primer, LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Sequence (A. th genome BLAST matches underlined) 
>66-K107480-SALK_087012C-LBb3
GGCAACCAGCGTGGACCGCTTGCTGCAACTCTCTCAGGGCCAGGCGGTGAAGGGCAATCA
GCTGTTGCCCGTCTCACTGGTGAAAAGAAAAACCACCCCAGTACATTAAAAACGTCCGCA
ATGTGTTATTAAGTTGTCTAAGCGTCAATTTGTTTACACCACAATATATTGTGGACAAAT
AATGTAAACAAACATATTAAATAGCTATGATTAAGATGTGAAATTGGTATATAATTATGA
AATACTTGAATTGGAAAATTGATATATACCTGATTGATCTTTTATTAGTGGACATGTGAA
AATTATCTTCAAAATTCCAATCAGCAAACACTTGTTCCATGTCAGATCTGTAAAGACACT
TAAATAAAGAATATATTACTTTTTTGTAAGTGAATAAGAAATGATTTGATTCATAATTAG
TATAATGATTAACAGGAGATTTGAGGAAAAAGGATGATGAAAGCAATCAACTTAGAAAAG
ATTATTCGAAATCCCAACAGTTTAATGATTAGAAAATAATTAAACCAAACAACCCTCTCC
AACAATCTAGGAGGAAATGATTTAATAAAGCAACTAATTCTATATATAGTAAACTCTTAC
TAAGGGGAAGCTATAAAAGTAATTACCAGATTTAGAAGCCTCTGAATGGATGGAAGATTT
AGC

Last Updated on 06.12.2018 16:11