Genotyping and primer details


Genotyping and primer details for SALK_087133 - At3g62180
Line ID SALK_087133
Locus At3g62180 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:23020320 of line SALK_087133 Chr3:23020320
   
Insertion allele amplicon LBb3 - 59qz
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 59qz: 5'-AATCATACATGACAGAACCAACCA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:23019731
   
wt amplicon (over InsPos) 14rz - 59qz
wt amplicon start Chr3:23019731
wt amplicon end Chr3:23020617
wt amplicon size 887 bp
Locus specific primer 59qz: 5'-AATCATACATGACAGAACCAACCA-3'
2nd locus specific primer 14rz: 5'-GCTAGCTGCCAAATATAGACGAAC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11