Sequences from confirmation amplicon


Confirmation sequences for line SALK_087133 and hit At3g62180 are shown.
Line ID SALK_087133
Locus At3g62180 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:23020320
Primers used for PCR 59qz - LBb3, show primer details
   
Sequence Name 76-K116055-SALK_087133C-59qz
GenBank Accession LN486158 [GenBank]
Sequencing primer gene specific primer, 59qz: 5'-AATCATACATGACAGAACCAACCA-3'
Sequence (A. th genome BLAST matches underlined) 
>76-K116055-SALK_087133C-59qz
CCTTACACGATATAATTAATTATTACATCCAATCCCTTAATACGATCATCAGTAATAATA
ATCAGACAAGGGTGGCGGTCGGGGCGAAGTGTCTTCCTCGTTAAGCTTCACCTTAATACC
AAAAGTCTTGAAAATAGTATTCAAGTTATTAAACACATCAAAAGAATCACTTGTTAGATT
CTTCCCAACTCCAATCCCAATCCCCACTTCCTTCAACAAAATCTTGTCTTCAATATCGTC
CAAACAAGTCGAATGATACCCCATGATTGACATTAACTCTTTTTTACACGTAAAATAATT
ATGAGCCAACGTCGTTACATCTTTCCCCGTAGCTTTCCAAAAATCGGCGAAATCTTCCAA
CGCGTAATTCAAGTTTTTCTCGCAGCTGTTGACCACTGCGGTTGCGGTTGCGTCTGACAT
GTGTTTTGGTTTGATTCCAGAGAGGAAAGTCACACTTTGTTTGACGGATGTTTCAGCGGC
AGCCACTAATGCTCGGATTAGTTCGATGGGGTCATTGGTAGGAACGTGTTTAAGGGTTTC
GCAGGATATATTGTGGTGTAAACAAATTGACGCTTAGACAACTTAATAACACATTGCGGA
CGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTG
CCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGC
AGGCGAAAATCCTGTTTGATGGTGGTTCCG
   
Sequence Name 49-K116054-SALK_087133C-R210
GenBank Accession LN485483 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>49-K116054-SALK_087133C-R210
CACAATATATCCTGCGACACCCTTAAACACGTTCCTACCAATGACACCATCGAACTAATC
CGAGCATTAGTGGCTGCCGCTGAAACATCCGTCAAACAAAGTGTGACTTTCCTCTCTGGA
ATCAAACCAAAACACATGTCAGACGCAACCGCAACCGCAGTGGTCAACAGCTGCGAGAAA
AACTTGAATTACGCGTTGGAATATTTCGCCGATTTTTGGAAAGCTACGGGGAAAGATGTA
ACGACGTTGGCTCATAATTATTTTACGTGTAAAAAAGAGTTAATGTCAATCATGGGGTAT
CATTCGACTTGTTTGGACGATATTGAAGACAAGATTTTGTTGAAGGAAGTGGGGATTGGG
ATTGGAGTTGGGAAGAATCTAACAAGTGATTCTTTTGATGTGTTTAATAACTTGAATACT
ATTTTCAAGACTTTTGGTATTAAGGTGAAGCTTAACGAGGAAGACACTTCGCCCCGACCG
CCACCCTTGACTGATTATTATTACTGATGATCGTATTAAGGGATTGGATGTAATAATTAA
TTATATCGTGTAAGGCCTAGAGCAATCGCGAATGGAATCATAGATGGTTGGTTCTGTCAT
GTATGATTACCCCCCCCCCCCCACAAAAAAAAA

Last Updated on 06.12.2018 16:11