Genotyping and primer details


Genotyping and primer details for SALK_087377c - At5g08100
Line ID SALK_087377c
Locus At5g08100 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:2594050 of line SALK_087377c Chr5:2594050
   
Insertion allele amplicon LBb3 - 02lv
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 02lv: 5'-GTCTTTAAGTTATACGGCGGAAGA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:2594609
   
wt amplicon (over InsPos) 07mc - 02lv
wt amplicon start Chr5:2593673
wt amplicon end Chr5:2594609
wt amplicon size 937 bp
Locus specific primer 02lv: 5'-GTCTTTAAGTTATACGGCGGAAGA-3'
2nd locus specific primer 07mc: 5'-GTACTTTCGGACTAGGGACTGTGT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11