Genotyping and primer details


Genotyping and primer details for SALK_087519c - At3g49880
Line ID SALK_087519c
Locus At3g49880 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:18496976 of line SALK_087519c Chr3:18496976
   
Insertion allele amplicon LBb3 - 15vt
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 15vt: 5'-CTTCGTTTTTCCATGTCCATAAAT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:18497604
   
wt amplicon (over InsPos) 68wi - 15vt
wt amplicon start Chr3:18496263
wt amplicon end Chr3:18497604
wt amplicon size 1342 bp
Locus specific primer 15vt: 5'-CTTCGTTTTTCCATGTCCATAAAT-3'
2nd locus specific primer 68wi: 5'-AGTATCATTCATCGGTCTCGTCTT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11