Genotyping and primer details


Genotyping and primer details for SALK_087753c - At1g31940
Line ID SALK_087753c
Locus At1g31940 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:11470381 of line SALK_087753c Chr1:11470381
   
Insertion allele amplicon LBb3 - 20rb
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 20rb: 5'-TCGGATTTGAAATACCCAGATTAG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:11470955
   
wt amplicon (over InsPos) 08se - 20rb
wt amplicon start Chr1:11470094
wt amplicon end Chr1:11470955
wt amplicon size 862 bp
Locus specific primer 20rb: 5'-TCGGATTTGAAATACCCAGATTAG-3'
2nd locus specific primer 08se: 5'-GGTGTTGTTGAGGTACATAGAGCA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11