Genotyping and primer details


Genotyping and primer details for SALK_087882c - At5g17810
Line ID SALK_087882c
Locus At5g17810 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:5880894 of line SALK_087882c Chr5:5880894
   
Insertion allele amplicon LBb3 - 83sm
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 83sm: 5'-CTGCAGAAATTAACCTCAAGAACC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:5881203
   
wt amplicon (over InsPos) 12tl - 83sm
wt amplicon start Chr5:5880429
wt amplicon end Chr5:5881203
wt amplicon size 775 bp
Locus specific primer 83sm: 5'-CTGCAGAAATTAACCTCAAGAACC-3'
2nd locus specific primer 12tl: 5'-GAAAGCAAAATATCTGACGAGTCC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11