Genotyping and primer details


Genotyping and primer details for SALK_088070c - At1g37130
Line ID SALK_088070c
Locus At1g37130 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:14160358 of line SALK_088070c Chr1:14160358
   
Insertion allele amplicon LBb3 - 37ux
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 37ux: 5'-TACGGTAATCCTCAAGCATCTTCT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:14160735
   
wt amplicon (over InsPos) 74vl - 37ux
wt amplicon start Chr1:14159840
wt amplicon end Chr1:14160735
wt amplicon size 896 bp
Locus specific primer 37ux: 5'-TACGGTAATCCTCAAGCATCTTCT-3'
2nd locus specific primer 74vl: 5'-TGTTTTAGGTTGGTGGTATAAGCC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11