Sequences from confirmation amplicon


Confirmation sequences for line SALK_088070c and hit At1g37130 are shown.
Line ID SALK_088070c
Locus At1g37130 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:14160358
Primers used for PCR 37ux - LBb3, show primer details
   
Sequence Name 29-K117668-SALK_088070C-R210
GenBank Accession LN485116 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>29-K117668-SALK_088070C-R210
CATTTGTTTGTGCAGCCACACAGGGAGAGATTGGGATTGTGTTCGAGCATCCAACGCTTC
CTGGTAATGAATCTGGTGGATGGATGGCGAAGGAACGTCACCTCGAAAAATCGGCTGACG
CGCCTCCTAGTCTAAAGAAGTCTGTCTCGACGCCGTTTATGAACACAACTGCGAAGATGT
ACTCGATGTCCGAGGTCAAGAAGCATAATTCGGCTGACTCTTGCTGGATCATTGTCCATG
GACATATCTATGATTGTACACGATTCCTTATGGATCACCCGGGTGGTTCGGATTCAATCT
TGATCAATGCTGGTACGGATTGTACGGAGGAGTTTGAAGCCATTCACTCGGATAAAGCCA
AGAAGATGCTTGAGGATTACCGT
   
Sequence Name 66-K117669-SALK_088070C-37ux
GenBank Accession LN485909 [GenBank]
Sequencing primer gene specific primer, 37ux: 5'-TACGGTAATCCTCAAGCATCTTCT-3'
Sequence (A. th genome BLAST matches underlined) 
>66-K117669-SALK_088070C-37ux
CTCAACTCCTCGTACATCCGTACCAGCATTGATCAAGATTGAATCCGAACCACCCGGGTG
ATCCATAAGGAATCGTGTACAATCATAGATATGTCCATGGACAATGATCCAGCAAGAGTC
AGCCGAATTATGCTTCTTGACCTCGGACATCGAGTACATCTTCGCAGTTGTGTTCATAAA
CGGCGTCGAGACAGACTTCTTTAGACTAGGAGGCGCGTCAGCCGATTTTTCGAGGTGACG
TTCCTTCGCCATCCATCCACCAGATTCATTACCAGGAAGCGTTGGATGCTCGAACACAAT
CCCAATCTCTCCCTTGTGTGGCTTGCACAAACAAATTGACGCTTAGACAACTTAATAACA
CATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAAC
AGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTT
TGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTCCGAATCNNGCAAAAT

Last Updated on 06.12.2018 16:11