Genotyping and primer details


Genotyping and primer details for SALK_088542c - At2g23380
Line ID SALK_088542c
Locus At2g23380 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:9959382 of line SALK_088542c Chr2:9959382
   
Insertion allele amplicon LBb3 - 36an
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 36an: 5'-CTGCTGCATATCATTCAATTAGGA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:9958711
   
wt amplicon (over InsPos) 06bs - 36an
wt amplicon start Chr2:9958711
wt amplicon end Chr2:9959585
wt amplicon size 875 bp
Locus specific primer 36an: 5'-CTGCTGCATATCATTCAATTAGGA-3'
2nd locus specific primer 06bs: 5'-GAGATCAGCTCTCCTGTGTATTCC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11