Genotyping and primer details

Genotyping and primer details for SALK_088888c - At5g40430
Line ID SALK_088888c
Locus At5g40430 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:16177030 of line SALK_088888c Chr5:16177030
Insertion allele amplicon LBb3 - 51iv
Locus specific primer 51iv: 5'-GTGATATTGTAGAAGCATCGGTCA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:16176516
wt amplicon (over InsPos) 14kj - 51iv
wt amplicon start Chr5:16176516
wt amplicon end Chr5:16177567
wt amplicon size 1052 bp
Locus specific primer 51iv: 5'-GTGATATTGTAGAAGCATCGGTCA-3'
2nd locus specific primer 14kj: 5'-AAGATGGACAGAATCTGAGGACAT-3'
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11