Genotyping and primer details


Genotyping and primer details for SALK_089556 - At3g56480
Line ID SALK_089556
Locus At3g56480 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:20937099 of line SALK_089556 Chr3:20937099
   
Insertion allele amplicon LBb3 - 43cp
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 43cp: 5'-GAGTTGAGGAATCCAAGTCTTGTC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:20937606
   
wt amplicon (over InsPos) 29dk - 43cp
wt amplicon start Chr3:20936882
wt amplicon end Chr3:20937606
wt amplicon size 725 bp
Locus specific primer 43cp: 5'-GAGTTGAGGAATCCAAGTCTTGTC-3'
2nd locus specific primer 29dk: 5'-TGTCGAATATAATTCTCTCGCCTT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11