Sequences from confirmation amplicon


Confirmation sequences for line SALK_089556 and hit At3g56480 are shown.
Line ID SALK_089556
Locus At3g56480 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:20937099
Primers used for PCR 43cp - LBb3, show primer details
   
Sequence Name 21-K108893-SALK_089556C-43cp
GenBank Accession LN484877 [GenBank]
Sequencing primer gene specific primer, 43cp: 5'-GAGTTGAGGAATCCAAGTCTTGTC-3'
Sequence (A. th genome BLAST matches underlined) 
>21-K108893-SALK_089556C-43cp
GGCTCTTGCTAAGAATCCGTGTCAATGCTCAAGACAATGCTCCAGATCTTTCCAACTGTT
CTATTCAGTGGTATCGTGCAGCATGTGAAACTAGTCGCAGGGAAGCTATATCTGGTAAAT
GGACCTATCTGTTTGTTTGCTTCATCATTTGGTTTTTAGTAAATGAATTTTAGCTTGAGC
AGTTCATATTCTTGCTCTTAGGTGCCATCCAATCGATGTATGCTCCAGAACCATTTGATG
TTGGGAGGATATTACAGGCAGACATTCTTTCAAATGGTCAAAAGTTCACAGTTACAACCG
ATGATCCAGTTGATCCTGGTTGGTATTACGGAAACTTTAGACGATACAAAGATTTTTAAG
TGGACTATAAGTGAATGTTTGGCTTACTGAAATGCAGATTCTGGCTTGCCATCCCGCGTA
GAGTCGCTGATGCGAAAGTCTAACAGTGAATTCACCAGTGAGACGGGCAACAGCTGATTG
CCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGC
AGGCGAAAATCCTGTTTGATGGTGGTTCCGAAAT
   
Sequence Name 05-K108892-SALK_089556C-R210
GenBank Accession queueing for submission to ENA/GenBank
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>05-K108892-SALK_089556C-R210
AAAAGGATGGCGCTGAAAAGCAGTGATTTGCATCCAAGGTTGGTTTTCCCAATTATAAAA
AA

Last Updated on 06.12.2018 16:11