Genotyping and primer details


Genotyping and primer details for SALK_089785c - At2g25230
Line ID SALK_089785c
Locus At2g25230 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:10747522 of line SALK_089785c Chr2:10747522
   
Insertion allele amplicon LBb3 - 37gz
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 37gz: 5'-TTTCAAAGGGTTATTAAAACGTGC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:10747838
   
wt amplicon (over InsPos) 12il - 37gz
wt amplicon start Chr2:10746969
wt amplicon end Chr2:10747838
wt amplicon size 870 bp
Locus specific primer 37gz: 5'-TTTCAAAGGGTTATTAAAACGTGC-3'
2nd locus specific primer 12il: 5'-TGTAAGTTGGGAAATTGTTGTTGA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11