Sequences from confirmation amplicon


Confirmation sequences for line SALK_089785c and hit At2g25230 are shown.
Line ID SALK_089785c
Locus At2g25230 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr2:10747522
Primers used for PCR 37gz - LBb3, show primer details
   
Sequence Name 76-K112564-SALK_089785C-37gz
GenBank Accession LN486150 [GenBank]
Sequencing primer gene specific primer, 37gz: 5'-TTTCAAAGGGTTATTAAAACGTGC-3'
Sequence (A. th genome BLAST matches underlined) 
>76-K112564-SALK_089785C-37gz
TAGTCATAGACATCAATAATAAGAGCATGATTAATTATTTATGTATATGTAAATGACATA
ATTACATATAAATAATTAATATGTGATTTCAGAAAACTGCTTGGAGTGAAGAAGAAGATC
AAATTTTGATTGAAGCACACAAAGTACTTGGTACCAAATGGGTCGAGATCGCACAGCAAC
TTCCGGGGAGGAGCGATAACAATATTAAGAACCATTGGAACACCACAAAGCGTCGGGTGC
AAAATAAAAGAGGTGGAAATATTGTGGTGTAAACAAATTGACGCTTAGACAACTTAATAA
CACATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCA
ACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGG
TTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTCCGAATCGGGCAAAA
   
Sequence Name 60-K112563-SALK_089785C-R210
GenBank Accession LN485742 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>60-K112563-SALK_089785C-R210
GTCATTTGTTACCCACAATATTTCACCTCTTTATTTTGCACCCGACGCTTTGTGGTGTTC
CAATGGTTCTTAATATTGTTATCGCTCCTCCCCGGAAGTTGCTGTGCGATCTCGACCCAT
TTGGTACCAAGTACTTTGTGTGCTTCAATCAAAATTTGATCTTCTTCTTCACTCCAAGCA
GTTTTCTGAAATCACATATTAATTATTTATATGTAATTATGTCATTTACATATACATAAA
TAATTAATCATGCTCTTATTATTGTATGTCTATGACTATTTTTATTGATAGAAGCAAGAT
ACAAACTAAAAAGATGCACGTTTTAATAACCCTTTGAA

Last Updated on 06.12.2018 16:11