Genotyping and primer details


Genotyping and primer details for SALK_089798c - At3g25520
Line ID SALK_089798c
Locus At3g25520 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:9269983 of line SALK_089798c Chr3:9269983
   
Insertion allele amplicon LBb3 - 12kt
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 12kt: 5'-TGATTCTGTCTTTATGGCTTAC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:9270526
   
wt amplicon (over InsPos) 10mc - 12kt
wt amplicon start Chr3:9269574
wt amplicon end Chr3:9270526
wt amplicon size 953 bp
Locus specific primer 12kt: 5'-TGATTCTGTCTTTATGGCTTAC-3'
2nd locus specific primer 10mc: 5'-TACTCTTCATCGTCCTCATCATCA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11