Genotyping and primer details


Genotyping and primer details for SALK_089912c - At1g72510
Line ID SALK_089912c
Locus At1g72510 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:27304350 of line SALK_089912c Chr1:27304350
   
Insertion allele amplicon LBb3 - 09tw
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 09tw: 5'-CAGAGATAAGCATGACCCTACCTT-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:27304798
   
wt amplicon (over InsPos) 07ue - 09tw
wt amplicon start Chr1:27303954
wt amplicon end Chr1:27304798
wt amplicon size 845 bp
Locus specific primer 09tw: 5'-CAGAGATAAGCATGACCCTACCTT-3'
2nd locus specific primer 07ue: 5'-GAGGTAATACTCGTCTCTCCGGTA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11