Sequences from confirmation amplicon


Confirmation sequences for line SALK_089912c and hit At1g72510 are shown.
Line ID SALK_089912c
Locus At1g72510 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr1:27304350
Primers used for PCR 09tw - LBb3, show primer details
   
Sequence Name 18-K116922-SALK_089912C-09tw
GenBank Accession LN484791 [GenBank]
Sequencing primer gene specific primer, 09tw: 5'-CAGAGATAAGCATGACCCTACCTT-3'
Sequence (A. th genome BLAST matches underlined) 
>18-K116922-SALK_089912C-09tw
AATTTCTAAAGAATCTCCTTCTTAATTATCTAATTATAATCGAATAGTATAAACATATTT
TCATTTCATTTTGTTCTTGTTTAACTAAGCTCACAGCAACAGAATTCCGAATATTAGCTA
GCAATTGGAGAAAGAATAAAAGACAGAAACAGAGCACCAAATTATTCTTTTAGCCCAAAA
TTTTCCAAAAGTACAACAACAAATCCGGCAAAAACACATACAGAAACCAATTTTTGATTT
ACCGACGAAAATTTCAAATCAGAAAACCGGATTTGATCCAAGTGGGTCCCTAAACCGACG
GTTTAAAACAATTTTTTCAACGATGAGCTTTTTTCAAGTCAAACTAGCGAAACAGCTATC
AGACCTAGACAAAACATTAGAAACACATTGATATATTGTGGTGTAAACAAATTGACGCCA
GGATATATTGTGGTGTAAACAAATTGACGCTTAGACAACTTAATAACACATTGCGGACGT
TTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGGGCAACAGCTGATTGCCC
TTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGCTGGTTTGCCCCAGCAGG
CGAAAATCCTGTTTGATGGTGGTTCCGA
   
Sequence Name 07-K116921-SALK_089912C-R210
GenBank Accession LN484470 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>07-K116921-SALK_089912C-R210
CACAATATATCCTGGCGTCAATTTGTTTACACCACAATATATCAATGTGTTTCTAATGTT
TTGTCTAGGTCTGATAGCTGTTTCGCTAGTTTGACTTGAAAAAAGCTCATCGTTGAAAAA
ATTGTTTTAAACCGTCGGTTTAGGGACCCACTTGGATCAAATCCGGTTTTCTGATTTGAA
ATTTTCGTCGGTAAATCAAAAATTGGTTTCTGTATGTGTTTTTGCCGGATTTGTTGTTGT
ACTTTTGGAAAATTTTGGGCTAAAAGAATAATTTGGTGCTCTGTTTCTGTCTTTTATTCT
TTCTCCAATTGCTAGCTAATATTCGGAATTCTGTTGCTGTGAGCTTAGTTAAACAAGAAC
AAAATGAAATGAAAATATGTTTATACTATTCGATTATAATTAGATAATTAAGAAGGAGAT
TCTTTAGAAATTTATCATATTGAAAAGACATGTATGATCAAAAACCATGGAAGGTAGGGT
CTGTTTAATCTCTG

Last Updated on 06.12.2018 16:11