Genotyping and primer details


Genotyping and primer details for SALK_090068 - At5g63630
Line ID SALK_090068
Locus At5g63630 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:25474425 of line SALK_090068 Chr5:25474425
   
Insertion allele amplicon LBb3 - 32mv
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 32mv: 5'-GATATTGGGGATTTGCTTAGTGAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:25475190
   
wt amplicon (over InsPos) 12oj - 32mv
wt amplicon start Chr5:25474347
wt amplicon end Chr5:25475190
wt amplicon size 844 bp
Locus specific primer 32mv: 5'-GATATTGGGGATTTGCTTAGTGAA-3'
2nd locus specific primer 12oj: 5'-GCTTGTAGGAGGAGATTTGACAAC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11