Genotyping and primer details


Genotyping and primer details for SALK_090362 - At3g18440
Line ID SALK_090362
Locus At3g18440 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:6328119 of line SALK_090362 Chr3:6328119
   
Insertion allele amplicon LBb3 - 51fm
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 51fm: 5'-AAAGAAGAAAACGACGAAAATTCA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:6328743
   
wt amplicon (over InsPos) 10go - 51fm
wt amplicon start Chr3:6327639
wt amplicon end Chr3:6328743
wt amplicon size 1105 bp
Locus specific primer 51fm: 5'-AAAGAAGAAAACGACGAAAATTCA-3'
2nd locus specific primer 10go: 5'-CAATTGTTTCCTCTGCTTAGCTTT-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11