Sequences from confirmation amplicon


Confirmation sequences for line SALK_090362 and hit At3g18440 are shown.
Line ID SALK_090362
Locus At3g18440 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:6328119
Primers used for PCR 51fm - LBb3, show primer details
   
Sequence Name 82-K111509-SALK_090362C-51fm
GenBank Accession LN486244 [GenBank]
Sequencing primer gene specific primer, 51fm: 5'-AAAGAAGAAAACGACGAAAATTCA-3'
Sequence (A. th genome BLAST matches underlined) 
>82-K111509-SALK_090362C-51fm
TCTTTCTTCACAAAGAAACAAACCAAACCCAGAAAATTCCAATCTAATAACTAAATTTCC
AATGAACCCCAAAATCGTGGAAAACTAAAACCTCAGGGGATGAAATTTACCGATGGTGAA
TTCGAAGACGACGACGACAGTAAGAATAGCCCAAACAGAGTATCGACTAAGATCCGGATT
AGGTTCCTGATAAAAAATCAAAAGCGCAACAATCGTCAGAGCAAGACCAATCTTAGCGGA
GAAGACGATCTTTCTCGGATCAGATACTCCCATCTCCCAAGCTTTCCTCGCTACATCTTT
AGCATCGTCGTAGACTCCAGATATTTTCTCAGATAGATTACCACACGAACAACAGCAACA
TAACCGTGTTCTTCGTCCACAGTTTTCGTTTTCGAGTAGATCGTTAGATTCGATGTCGGT
GAATCTGAAATCGGAGAAACCATTATTGGAGAGAAGCCTCTCCCGTTTCTCGAGTATTCC
ATGCCTGAAGGAACCTTGCTTCGCCGCCATTACTAGACGGATTCTCAAAGAGAATTAAGC
TTTGTTTGAAAAAAGAATGGTGAAGATATTTTTTATTTGACTGACGCTTAGACAACTTAA
TAACACATTGCGGACGTTTTTAATGTACTGGGGTGGTTTTTCTTTTCACCAGTGAGACGG
GCAACAGCTGATTGCCCTTCACCGCCTGGCCCTGAGAGAGTTGCAGCAAGCGGTCCACGC
TGGTTTGCCCCAGCAGGCGAAAATCCTGTTTGATGGTGGTTCC
   
Sequence Name 68-K111508-SALK_090362C-R210
GenBank Accession LN485952 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>68-K111508-SALK_090362C-R210
CAATAAAATATCTTCACCATTCTTTTTTCAACAAAGCTTAATTCTCTTTGAGAATCCGTC
TAGTAATGGCGGCGAAGCAAGGTTCCTTCAGGCATGGAATACTCGAGAAACGGGAGAGGC
TTCTCTCCAATAATGGTTTCTCCGATTTCAGATTCACCGACATCGAATCTAACGATCTAC
TCGAAAACGAAAACTGTGGACGAAGAACACGGTTATGTTGCTGTTGTTCGTGTGGTAATC
TATCTGAGAAAATATCTGGAGTCTACGACGATGCTAAAGATGTAGCGAGGAAAGCTTGGG
AGATGGGAGTATCTGATCCGAGAAAGATCGTCTTCTCCGCTAAGATTGGTCTTGCTCTGA
CGATTGTTGCGCTTTTGATTTTTTATCAGGAACCTAATCCGGATCTTAGTCGATACTCTG
TTTGGGCTATTCTTACTGTCGTCGTCGTCTTCGAATTCACCATCGGTAAATTTCATCCCC
TGAGGTTTTAGTTTTCCACGATTTTGGGGTTCATTGGAAATTTAGTTATTAGATTGGAAT
TTTCTGGGTTTGGTTTGTTTCTTTGTTGAAGAAAGAGTATGCGTCGTTGCTTTCATCTGT
AGAATGAATTTTCGTCGTTNTCTTCTTT

Last Updated on 06.12.2018 16:11