Genotyping and primer details


Genotyping and primer details for SALK_091291c - At2g45140
Line ID SALK_091291c
Locus At2g45140 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr2:18612159 of line SALK_091291c Chr2:18612159
   
Insertion allele amplicon LBb3 - 63fr
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 63fr: 5'-TCTTTGATTCTTAAACCAACCACC-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr2:18612670
   
wt amplicon (over InsPos) 01hv - 63fr
wt amplicon start Chr2:18611853
wt amplicon end Chr2:18612670
wt amplicon size 818 bp
Locus specific primer 63fr: 5'-TCTTTGATTCTTAAACCAACCACC-3'
2nd locus specific primer 01hv: 5'-ATGGGAATGCTTCTGATTTTACTG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11