Genotyping and primer details


Genotyping and primer details for SALK_092421c - At3g61190
Line ID SALK_092421c
Locus At3g61190 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:22650657 of line SALK_092421c Chr3:22650657
   
Insertion allele amplicon LBb3 - 40fp
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 40fp: 5'-AACAATGATGACAAAAACATTGGA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:22651377
   
wt amplicon (over InsPos) 08go - 40fp
wt amplicon start Chr3:22650579
wt amplicon end Chr3:22651377
wt amplicon size 799 bp
Locus specific primer 40fp: 5'-AACAATGATGACAAAAACATTGGA-3'
2nd locus specific primer 08go: 5'-TACAAACCAGAACAATTGCAAGAC-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11