Sequences from confirmation amplicon


Confirmation sequences for line SALK_092421c and hit At3g61190 are shown.
Line ID SALK_092421c
Locus At3g61190 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Chr3:22650657
Primers used for PCR 40fp - LBb3, show primer details
   
Sequence Name 83-K111509-SALK_092421C-40fp
GenBank Accession LN486257 [GenBank]
Sequencing primer gene specific primer, 40fp: 5'-AACAATGATGACAAAAACATTGGA-3'
Sequence (A. th genome BLAST matches underlined) 
>83-K111509-SALK_092421C-40fp
GCTTAGCTAACCGGAGACCCATCAAGAAAAGACTTTCGCCGTCGTAAAAATCGACGAGAA
ATGTCGAAAATCTAATCTTGACGAATCGAGAAGAAGCAATCCTACATGGAACTATAAATC
AGAGATGCCCATCAATGGTAATGAACAGTTTATCTTCATTGAGGTATTTTATCGAACAGG
TTCTGGGCATGATAAAAAAATCGGAGAAGCTAAAATCCCAACGAATGATTTCATGGGAAG
GTATTCACCTGAAGGTCACTTAAACTTCTTGAGCTATAGATTGAGAGATGAGTTTGGTGA
TAAGTGTGGGATCGTCAATCTTTCGATTTTGGTTAAATCGGATCCCACCAGAGATTACGG
CGCGTGTTCGTCCCAAGCGGCGGTTACTGGTTTGTGGAGGCCGAGATTAGAGACGGCATC
GATTGATGGATACGGTGGCCGGACTGTTACCGGAGTTCCGGTTTGGGGTCTGTATCAACG
CCNATTTTAAAATCTTTTTTTTTTGGGGGGGGATGCACATATAATTAATTCTTCGTCTTT
GTTCCATAAATTTTCCCAATTTGGAATTGAGGAACAAAAATGTGTGATTAATTTTTGTTA
TAATACTCACAAAACCAAAATTGAAAATAGAAAAGTCAAATTTGTTCAACAATAATTTCT
AAAAACTTGTTGTTATATGGTGGACAACTTAATAACACATTGCGAACGTTTTTAATGTAC
TGGGGTGGTTTTTCTTTTCACCATTGAGACGGGCAACAGCTGATTGCCCTTCACCGCCGG
GCCC
   
Sequence Name 69-K111508-SALK_092421C-R210
GenBank Accession LN485976 [GenBank]
Sequencing primer T-DNA primer, R210: 5'-CCCAGTACATTAAAAACGTCCGC-3'
Sequence (A. th genome BLAST matches underlined) 
>69-K111508-SALK_092421C-R210
TATACACAAGTTCTTAATATCATCGTCGAACAAATTTGACTTTTCTATTTTCAATTTTGG
TTTCGTGAGTATTATAACAAAAATTAATCACACATCTTTGTTCCTCAATTCCAAATTGGG
AAAATCTATGGAACAAAGACGAAGAATTAATAATATGTGCATCCCCCCCCAAAAAAAAAA
GATTTTAAAATTGGCGTTGATACAGACCCCAAACCGGAACTCCGGTAACAGTCCGGCCAC
CGTATCCTTCAATCGATGCCGTCTCTAATCTCGGCCTCCACAAACCAGTAACCGCCGCTG
GGGACAAACACGCGCCGTAATCTCTGGTGGGATCCGATTTAACCAAAATCGAAAGATTGA
CAATCCCACACTTATCACCAAACTCATCTCTCAATCTATAGCTCAAGAAGTTTAAGTGAC
CTTCAGGTGAATACCTTCCCATGAAATCATTCGTTGGGATTTTAGCTTCTCCAATTTTTT
TATCAGGCCCAAAACCTGTTCAATAAAATACCTCAATGAAAATAAACTGTTCATTACCAT
TGATGGGCATCTCTAATTTATAGTTCCATGTAGGATTGCTTCTTCTCGATTCGTCAAGAT
AAAATTTTCAACATTTCTCGTCAATTTTTACAACGGCAAAGGTCTTTTTCTTGATGGGTC
TCCGGTTTAGCTAAAGCCCTTCTGCTGATCTCAAATCAATCTCCAATGTTTTTGT

Last Updated on 06.12.2018 16:11