Genotyping and primer details


Genotyping and primer details for SALK_092571c - At5g25350
Line ID SALK_092571c
Locus At5g25350 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr5:8795096 of line SALK_092571c Chr5:8795096
   
Insertion allele amplicon LBb3 - 38dr
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 38dr: 5'-ATCTGAAGCATGTCTCTCTGAACA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr5:8795659
   
wt amplicon (over InsPos) 09ep - 38dr
wt amplicon start Chr5:8794720
wt amplicon end Chr5:8795659
wt amplicon size 940 bp
Locus specific primer 38dr: 5'-ATCTGAAGCATGTCTCTCTGAACA-3'
2nd locus specific primer 09ep: 5'-TGCAGTGTCCTGAGAAAGAAACTA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11