Genotyping and primer details


Genotyping and primer details for SALK_092885 - At1g20350
Line ID SALK_092885
Locus At1g20350 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:7044096 of line SALK_092885 Chr1:7044096
   
Insertion allele amplicon LBb3 - 12ei
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 12ei: 5'-ATGGCTCTAAAAGCAAACTCCATA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:7043394
   
wt amplicon (over InsPos) 22gc - 12ei
wt amplicon start Chr1:7043394
wt amplicon end Chr1:7044344
wt amplicon size 951 bp
Locus specific primer 12ei: 5'-ATGGCTCTAAAAGCAAACTCCATA-3'
2nd locus specific primer 22gc: 5'-ATGTGAGTTCTGCTCTCTGAATGA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11