Genotyping and primer details


Genotyping and primer details for SALK_093085c - At1g50400
Line ID SALK_093085c
Locus At1g50400 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:18668968 of line SALK_093085c Chr1:18668968
   
Insertion allele amplicon LBb3 - 52fm
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 52fm: 5'-GAGGCAACCTGTTGGATAATAAAA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:18669758
   
wt amplicon (over InsPos) 07go - 52fm
wt amplicon start Chr1:18668658
wt amplicon end Chr1:18669758
wt amplicon size 1101 bp
Locus specific primer 52fm: 5'-GAGGCAACCTGTTGGATAATAAAA-3'
2nd locus specific primer 07go: 5'-TTTAGGTTAATTTGTGGCACATCA-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11