Genotyping and primer details


Genotyping and primer details for SALK_093414 - At1g53280
Line ID SALK_093414
Locus At1g53280 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr1:19866221 of line SALK_093414 Chr1:19866221
   
Insertion allele amplicon LBb3 - 34ea
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 34ea: 5'-TTGAGTCAAGAGTGGAAATTGATG-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr1:19866508
   
wt amplicon (over InsPos) 17fg - 34ea
wt amplicon start Chr1:19865423
wt amplicon end Chr1:19866508
wt amplicon size 1086 bp
Locus specific primer 34ea: 5'-TTGAGTCAAGAGTGGAAATTGATG-3'
2nd locus specific primer 17fg: 5'-CGAGACCACCCTGATAATAAAAAG-3'
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11