Genotyping and primer details


Genotyping and primer details for SALK_093721 - At3g52700
Line ID SALK_093721
Locus At3g52700 [Araport] [TAIR] [MIPS] [SIGnAL]
Deduced position of insertion Graphic view of Chr3:19535480 of line SALK_093721 Chr3:19535480
   
Insertion allele amplicon LBb3 - 04tj
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 04tj: 5'-AGGACAAAAAGCGAAACTAATTCA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:19535810
   
Insertion allele amplicon LBb3 - 12bb
T-DNA primer LBb3: 5'-ATTTTGCCGATTTCGGAAC-3'
Locus specific primer 12bb: 5'-AGGACAAAAAGCGAAACTAATTCA-3'
Sequences from confirmation amplicon show confirmation sequences
Genomic end of ins. amplicon Chr3:19535810
   
Note The data concerning the primers refer to the experimental conditions used in the initial analysis of the alleles. However, in the course of the project we replaced the primer R204 with LBb3. See the DUPLOdb help page for details.

Last Updated on 06.12.2018 16:11